genechip mouse clariom s microarrays (Thermo Fisher)
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Genechip Mouse Clariom S Microarrays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Transcriptome profiling of macrophages persistently infected with human respiratory syncytial virus and effect of recombinant Taenia solium calreticulin on immune-related genes"
Article Title: Transcriptome profiling of macrophages persistently infected with human respiratory syncytial virus and effect of recombinant Taenia solium calreticulin on immune-related genes
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2024.1402589
Figure Legend Snippet: Time-dependent gene expression in persistent infection of hRSV versus niMϕ. (A) Heatmap representation of differentially expressed genes in piMϕ versus niMϕ after 6 h of in vitro culture. (B) Number of altered genes in piMϕ at 6 h of in vitro culture. (C) Heatmap representation of differentially expressed genes in piMϕ versus niMϕ after 24 h of in vitro culture. (D) Number of altered genes in piMϕ at 24 h of in vitro culture. (E) Venn diagram of genes expressed only at 6 or 24 h and at both timepoints. Top-up-regulated genes are shown in red, while top-down-regulated genes are shown in blue. Common genes expressed at both timepoints are shown in black. (F) Microarray expression data validation by qRT-PCR at 6 and 24 h. piMϕ , persistently infected macrophages with hRSV; niMϕ , non-infected macrophages; hRSV, human respiratory syncytial virus. p -values were calculated using the Student t -test between microarrays and rt-PCR data for each cytokine: *** = ≤0.001 and **** = ≤0.0001.
Techniques Used: Gene Expression, Infection, In Vitro, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, Virus, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Time-dependent gene expression in niMϕ treated with 5 μg of recombinant T. solium calreticulin (rTsCRT + ). (A) Heatmap representation of differentially expressed genes in niMϕ after rTsCRT treatment for 6 h. (B) Number of altered genes in niMϕ after rTsCRT treatment for 6 h. (C) Heatmap representation of differentially expressed genes in niMϕ after rTsCRT treatment for 24 h. (D) Number of altered genes in niMϕ after rTsCRT treatment for 24 h. (E) Venn diagram of the genes expressed only at 6 or 24 h and at both timepoints. Top-up-regulated genes are shown in red, while top-down-regulated genes are shown in blue. Common genes expressed at both timepoints are shown in black. (F) Microarray expression data validation by qRT-PCR at 6 and 24 h. (G) Temporary gene expression of niMϕ treated with rTsCRT. niMϕ , non-infected macrophages. p -values were calculated using the Student t -test between microarrays and rt-PCR data for each cytokine: * = <0.05, ** = <0.01, and *** = ≤0.001.
Techniques Used: Gene Expression, Recombinant, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, Infection, Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Time-dependent gene expression in piMϕ treated with 5 μg of recombinant T. solium calreticulin (rTsCRT + ). (A) Heatmap representation of differentially expressed genes in piMϕ after rTsCRT treatment for 6 h. (B) Number of altered genes in piMϕ after rTsCRT treatment for 6 h. (C) Heatmap representation of differentially expressed genes in piMϕ after rTsCRT treatment for 24 h. (D) Number of altered genes in piMϕ after rTsCRT treatment for 24 h. (E) Venn diagram of the genes expressed only at 6 or 24 h and at both timepoints. Top up-regulated genes are shown in red, while top down-regulated genes are in shown blue. Common genes expressed at both times are in black. (F) Microarray expression data validation by qRT-PCR at 6 and 24 h (G) Temporary gene expression of piMϕ treated with rTsCRT. piMϕ , persistently infected macrophages with hRSV; hRSV, human respiratory syncytial virus. p -values were calculated using the Student t -test between microarrays and rt-PCR data from each cytokine: * = <0.05, ** = <0.01, and *** = ≤0.001.
Techniques Used: Gene Expression, Recombinant, Microarray, Expressing, Biomarker Discovery, Quantitative RT-PCR, Infection, Virus, Reverse Transcription Polymerase Chain Reaction